Fabrication of a Human Lamina Cribrosa Mimic by Co-electrospinning
نویسندگان
چکیده
leaves only the biomaterial and hollow channels; SEM of gelatin with extracted PCL (magnification 1.50 KX, scale bar 20 μm) (c); Confocal fluorescent image of a DRG stained with phalloidin rhodamine (actin) after 4 days on the scaffold consisting of gelatin, elastin, and hollow channels (magnificiation 10X, scale bar 200 μm (d); Average aspect ratio of the DRGs on various fibrous scaffolds after 4 days (e). FABRICATION OF A HUMAN LAMINA CRIBROSA MIMIC BY CO-ELECTROSPINNING J. Hochstein, M. S. Earley, A. H. Rajabi Zamani, G. Collins, B. Mantilla, and M. Jaffe New Jersey Institute of Technology, Newark, NJ, Johns Hopkins University, Baltimore, MD, Virginia Tech, Blacksburg, VA
منابع مشابه
Age related compliance of the lamina cribrosa in human eyes.
AIMS To investigate changes in the mechanical compliance of ex vivo human lamina cribrosa with age. METHODS A laser scanning confocal microscope was used to image the surface of the fluorescently labelled lamina cribrosa in cadaver eyes. A method was developed to determine changes in the volume and strain of the lamina cribrosa created by increases in pressure. The ability of the lamina cribr...
متن کاملCell adhesion glycoproteins in the human lamina cribrosa.
PURPOSE The distribution of the cell adhesion glycoproteins, laminin, fibronectin, tenascin, vitronectin, thrombospondin, and entactin/nidogen, was examined in the human lamina cribrosa. METHODS Frozen sections of the optic nerve head from 7 normal human elderly donors were stained by immunohistochemistry. RESULTS All six glycoproteins were detected in this tissue. While laminin and entacti...
متن کاملMorphometry of the human lamina cribrosa surface.
The lamina cribrosa is a sieve-like perforation in the posterior part of the sclera, that allows passage of the retinal ganglion cell axons and central retinal vessels and preserves a pressure gradient between the intraocular and extraocular space. It has been termed the primary site of glaucomatous damage to the optic nerve. Using electron microscopy, the authors morphometrically evaluated the...
متن کاملCentral corneal thickness and thickness of the lamina cribrosa in human eyes.
PURPOSE Since central corneal thickness may inversely influence the amount and rate of progression of glaucomatous optic nerve damage and because lamina cribrosa thickness may be of importance in susceptibility to glaucoma, it was the purpose of the present study to evaluate whether central corneal thickness is related to lamina cribrosa thickness. METHODS The histomorphometric study included...
متن کاملFinite element modeling of optic nerve head biomechanics.
PURPOSE Biomechanical factors have been implicated in the development of glaucomatous optic neuropathy, particularly at the level of the lamina cribrosa. The goal of this study was to characterize the biomechanics of the optic nerve head using computer modeling techniques. METHODS Several models of the optic nerve head tissues (pre- and postlaminar neural tissue, lamina cribrosa, central reti...
متن کامل